Bone Cultures
نویسندگان
چکیده
منابع مشابه
Long-term human bone marrow cultures.
Pronlonged in vitro growth of murine bone marrow has been achieved using a flask culture system. We report the adaption of the technique to the growth of human bone marrow. In this system, CFU-GM and CFU-E are maintained for 4-9 wk. Morphologically recognizable granulopoiesis occurs for 4-6 wk and erythropoiesis for 1.5-2.5 wk. Functional T lymphocytes are maintained for at least 5 wk. The adhe...
متن کاملInduction of Mineralized Nodule Formation in Rat Bone Marrow Stromal Cell Cultures by Silk Fibroin
Background: Silk fibroin is a suitable protein for osteogenesis by inducing markers of bone formation in the cultures of osteoblasts, so we examined the ability of this protein to induce mineralized nodules in the rat bone marrow stromal cell cultures. Methods: Bone marrow stromal cells obtained from 4 to 6 weeks old Spruge-Dawely male rats were grown in primary culture for seven days and then ...
متن کاملOxygen tension measurements in bone marrow fleck cultures.
Cultures-Marrow fluid was aspirated from the sternum, ilium or vertebral spine and placed in a tube containing 1 mg. of heparin dissolved in 1 ml. of normal serum. After pouring it into a petri dish, the separated flecks of marrow w’re cultured as described dl’ Fifty marrow flecks from 7 individuals were cultured, and measurements of oxygen tension in the fluid medium were taken at intervals. C...
متن کاملCellular Activities in Tissue Cultures of Leukemic Human Bone Marrow.
Bone marrow specimens were obtained by aspiration from the sternum. Two to 3 ml. of aspirate were injected into a test tube containing 9 ml. of tissue culture medium and 1 ml. of heparin ( Liquaemin heparin, Organon ) . Bone marrow particles were collected with a capillary pipette from the sediment and from the fatty surface layer. The bone marrow particles were transferred into a second and th...
متن کاملAnalysis of megakaryocyte ploidy in rat bone marrow cultures.
Megakaryocytes undergo changes in ploidy in vivo in response to varying demands for platelets. Attempts to study the putative factor(s) regulating these ploidy changes have been frustrated by the lack of an appropriate in vitro model of megakaryocyte endomitosis. This report describes a culture system in which rat bone marrow is depleted of identifiable megakaryocytes and enriched in their prec...
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ژورنال
عنوان ژورنال: American Journal of Hospital Medicine
سال: 2019
ISSN: 2474-7017
DOI: 10.24150/ajhm/2019.012